primary human pulmonary microvascular endothelial cells (PromoCell)
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PromoCell
primary human pulmonary microvascular endothelial cells
Primary Human Pulmonary Microvascular Endothelial Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pulmonary+microvascular+endothelial+cells/Human+Pulmonary+Microvascular+Endothelial+Cells/pmc13621675-43-6-13
Average 96 stars, based on 166 article reviews
Primary Human Pulmonary Microvascular Endothelial Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pulmonary+microvascular+endothelial+cells/Human+Pulmonary+Microvascular+Endothelial+Cells/pmc13621675-43-6-13
Average 96 stars, based on 166 article reviews
primary human pulmonary microvascular endothelial cells - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "The Tie-2 agonist PMC-403 inhibits endothelial damage induced by e-cigarette vapor or LPS"
Article Title: The Tie-2 agonist PMC-403 inhibits endothelial damage induced by e-cigarette vapor or LPS
Journal: Respiratory Research
doi: 10.1186/s12931-026-03928-4
Figure Legend Snippet: A A schematic setup is shown for stimulation with the Tie2 agonist PMC-403 and its effects on HUVECs and HPMECs, accordingly. Created in BioRender. Stahl, K. (2026) https://BioRender.com/yc7s3fj . B HUVECs were stimulated with different concentrations of the Tie2 agonist PMC-403 or 500 ng/ml Angpt-1 for 1 hour. A western blot was performed and pAKT and AKT were analyzed. C HPMECs were incubated with 1 µg/ml PMC-403 for 1 hour and then stimulated with LPS. TER was measured by ECIS over time and group differences over time were analyzed by a linear mixed-effects model accordingly. D Group differences are shown 6.5 hours after LPS stimulation. Results are shown as dot plots with mean ± SD and each group consisted of n =4. P -values < 0.05 are considered significant. Abbreviations: Angpt-1 - Angiopoietin-1, ECIS - Electric cell-substrate impedance sensing, HPMECs - human pulmonary microvascular endothelial cells, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance
Techniques Used: Western Blot, Incubation, Electric Cell-substrate Impedance Sensing, Standard Deviation
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Derivative Assay:Article Title: Stable Knock-Down of the Sphingosine 1-Phosphate Receptor S1P1Influences Multiple Functions of Human Endothelial Cells Article Snippet: HUVECs were isolated as described26 and propagated in ECG medium (PromoCell). .. The human cell lines derived from human Cell Culture:Article Title: Stable Knock-Down of the Sphingosine 1-Phosphate Receptor S1P1Influences Multiple Functions of Human Endothelial Cells Article Snippet: HUVECs were isolated as described26 and propagated in ECG medium (PromoCell). .. The human cell lines derived from human Article Title: Circulating hyaluronic acid signature in CAP and ARDS - the role of pneumolysin in hyaluronic acid shedding. Article Snippet: Shedding of hyaluronan (HA), the component of endothelial cell (EC) glycocalyx, has been associated with acute lung injury.. HA degradation allows plasma proteins and fluid to penetrate across the vascular wall leading to lung edema formation and leukocyte recruitment.. Here, we analyzed sHA levels and size in patients with community-acquired pneumonia (CAP) and acute respiratory distress syndrome (ARDS), correlated them to disease severity, and evaluated the impact of pneumolysin (PLY), the Streptococcus pneumoniae (S.p.) exotoxin, on HA shedding from human pulmonary microvascular EC (HPMVEC). sHA levels were elevated in CAP and ARDS and correlated with the CRB65 severity score and with markers of inflammation (interleukin-6), EC activation (E-selectin), and basement membrane destruction (collagen IV). Isolation:Article Title: Aging-regulated TUG1 is dispensable for endothelial cell function. Article Snippet: Total RNA was isolated after 48 h with QIazol (Qiagen) and the Direct-zol RNA miniprep kit (Zymo Research) according to the manufacturer’s instructions including DNase digest. .. Alternatively, total RNA was isolated from cell pellets from cardiomyocytes, aortic fibroblasts, pericytes, aortic smooth muscle cells, mesenchymal stem cells, dermal lymphatic endothelial cells, umbilical vein endothelial cells, saphenous vein endothelial cells, Article Title: Aging-regulated TUG1 is dispensable for endothelial cell function Article Snippet: Total RNA was isolated after 48 h with QIazol (Qiagen) and the Direct-zol RNA miniprep kit (Zymo Research) according to the manufacturer’s instructions including DNase digest. .. Alternatively, total RNA was isolated from cell pellets from cardiomyocytes, aortic fibroblasts, pericytes, aortic smooth muscle cells, mesenchymal stem cells, dermal lymphatic endothelial cells, umbilical vein endothelial cells, saphenous vein endothelial cells, Article Title: Aging-regulated TUG1 is dispensable for endothelial cell function Article Snippet: Total RNA was isolated after 48 h with QIazol (Qiagen) and the Direct-zol RNA miniprep kit (Zymo Research) according to the manufacturer’s instructions including DNase digest. .. Alternatively, total RNA was isolated from cell pellets from cardiomyocytes, aortic fibroblasts, pericytes, aortic smooth muscle cells, mesenchymal stem cells, dermal lymphatic endothelial cells, umbilical vein endothelial cells, saphenous vein endothelial cells, Electric Cell-substrate Impedance Sensing:Article Title: Bone marrow donor selection and characterization of MSCs is critical for pre-clinical and clinical cell dose production Article Snippet: Bright field images were captured throughout culture and following each stain (EVOS digital inverted microscope, Life Technologies and Leica CTR6500, JH Technologies). .. The barrier integrity of human Article Title: Heparan sulfate mimetic fucoidan restores the endothelial glycocalyx and protects against dysfunction induced by serum of COVID-19 patients in the intensive care unit. Article Snippet: Endothelial barrier function assay Endothelial barrier function analysis was performed with impedance-based cell monitoring using the electric cell-substrate impedance sensing system (ECIS Zθ; Applied Biophysics, New York, NY, USA). .. ECIS plates (96W20idf PET; Applied Biophysics) were pre-treated with 10 mM L-cysteine and coated with 1% gelatine for primary human glomerular microvascular endothelial cells (GEnCs; ACBRI-128; Cell Systems, Kirkland, WA, USA) or without 1% gelatine for primary Positron Emission Tomography:Article Title: Heparan sulfate mimetic fucoidan restores the endothelial glycocalyx and protects against dysfunction induced by serum of COVID-19 patients in the intensive care unit. Article Snippet: Endothelial barrier function assay Endothelial barrier function analysis was performed with impedance-based cell monitoring using the electric cell-substrate impedance sensing system (ECIS Zθ; Applied Biophysics, New York, NY, USA). .. ECIS plates (96W20idf PET; Applied Biophysics) were pre-treated with 10 mM L-cysteine and coated with 1% gelatine for primary human glomerular microvascular endothelial cells (GEnCs; ACBRI-128; Cell Systems, Kirkland, WA, USA) or without 1% gelatine for primary |
